It hosts the Royal Bahraini Air Force (RBAF) Fighter Wing and the two squadrons that comprise it.
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Fighter aircraft are stored in 10 buildings located at either end of the runway.
In March 2009, the United States Air Force established a camp on Isa Air Base to support aerial port operations. The unit was designated a detachment of the 379th Air Expeditionary Wing of Al Udeid Air Base, Qatar. This detachment's purpose was to accelerate the delivery of mine-resistant, ambush-protected all-terrain vehicles to U.S. forces in Afghanistan.